Physical map location of the new Escherichia coli gene sbm

Location of the rpoN gene on the physical map of Escherichia coli.

Location of the prlC (opdA) gene on the physical map of Escherichia coli.

Location of the ubiA gene on the physical map of Escherichia coli.

We have determined the physical map position and the entire nucleotide sequence of the ubi4 gene. The sequence includes the structural gene for 4-hydroxybenzoate octaprenyltransferase, an enzyme involved in the early steps of the biosynthesis of ubiquinone in Eschenichia coli K-12. As shown in Fig. 1, clones 1F8 (634) and 12B4 (635) of Kohara's library (2) complemented an authentic ubi4 mutant ...

Physical map location of the peptide methionine sulfoxide reductase gene on the Escherichia coli chromosome.

The enzyme peptide methionine sulfoxide reductase (MsrA), the product of the msrA gene, catalyzes the reduction of methionine sulfoxide residues in proteins to methionine (1, 3, 4). During aerobic metabolism, methionine in proteins may be oxidized to methionine sulfoxide by biological reagents such as hydrogen peroxide, hydroxyl radicals, and hypochlorite and superoxide ions (reviewed in refere...

Physical map location of the Escherichia coli gene encoding acyl coenzyme A synthetase.

E. coli contains a single acyl-CoA synthetase, which has been purified to homogeneity (3, 4). In the process of long-chain fatty acid transport, this enzyme plays a pivotal role by catalyzing the thioesterification of exogenous fatty acids into metabolically active CoA thioesters prior to 3-oxidation concomitant with transport. This enzyme has broad chain-length specificity, giving Vm. values r...